Author Affiliations
Abstract
1 Department of Electronic and Computer Engineering, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
2 Department of Physics, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
Gallium nitride (GaN)-based light-emitting diodes (LEDs) are important for lighting and display applications. In this paper, we demonstrate green-emission (512 nm) InGaN quantum dot (QD) LEDs grown on a c-plane sapphire substrate by metal-organic chemical vapor deposition. A radiative lifetime of 707 ps for the uniform InGaN self-assembled QDs is obtained by time-resolved photoluminescence measurement at 18 K. The screening of the built-in fields in the QDs effectively improves the performance of QD LEDs. These high quantum efficiency and high temperature stability green QD LEDs are able to operate with negligible efficiency droop and with current density up to . Our results show that InGaN QDs may be a viable option as the active medium for stable LEDs.
Photonics Research
2020, 8(5): 05000750
1 中国中医科学院西苑医院， 北京 100091
2 中国中医科学院医学实验中心， 北京 100700
比较两种荧光探针异硫氰酸荧光素FITC和Cy5标记鹿茸提取物PAEs的最佳方法。用FITC和Cy5分别标记PAEs得到标记物FITC-PAEs和Cy5-PAEs; 用SDS-PAGE凝胶电泳检测并比较两种荧光染料标记蛋白分子量的差异; 用酶标仪检测在特定波长处OD值计算其标记率; 用PC12细胞验证两种荧光染料的生物安全性; 将荧光标记物分别与人工胃液和人工肠液共孵育，观察它们在其中的稳定性。结果表明，两种荧光染料标记PAEs蛋白分子量并无明显差异，但FITC-PAEs荧光强度稍强。通过计算标记率表明两种荧光染料均可充分标记PAEs，但Cy5价格昂贵。相比于FITC-PAEs，Cy5-PAEs在人工胃液和人工肠液中稳定性较差。因此，我们选择标记率高，在人工胃液和人工肠液中较为稳定且对机体无毒副作用，价格合理的FITC荧光染料标记PAEs并用于后续实验研究。这为建立蛋白类中药大分子的活性示踪方法，开发口服蛋白类药物奠定基础。
鹿茸提取物 荧光标记技术 异硫氰酸荧光素 pilose anlter extracts fluorescently labeled tracer technique FITC Cy5 Cy5
